Table 1. MLST alleles, GenBank accession numbers, PCR and sequencing primers (5′ to 3′) for blood samples No. 2 & 3.
Gene | MLST Allele | Genbank | PCR Forward 5′-3′ | PCR Reverse 5′-3′ |
aspA | 24 | EU056829 | AGTACTAATGATGCTTATCC | ATTTCATCAATTTGTTCTTTGC |
glnA | 2 | EU056830 | TAGGAACTTGGCATCATATTACC | TTGGACGAGCTTCTACTGGC. |
gltA | 2 | EU056831 | GGGCTTGACTTCTACAGCTACTTG | CCAAATAAAGTTGTCTTGGACGG |
glyA | 15 | EU056832 | GAGTTAGAGCGTCAATGTGAAGG | AAACCTCTGGCAGTAAGGGC |
pgm | 294 | EU056833 | GCAAACTCAGGACACCCAGG | AAAGCATTGTTAATGGCTGC |
tkt | 3 | EU056834 | GCTTAGCAGATATTTTAAGTG | AAGCCTGCTTGTTCTTTGGC |
uncA | 12 | EU056835 | ATGGACTTAAGAATATTATGGC | ATAAATTCCATCTTCAAATTCC. |
C. jejuni 16S rRNA | N/A | EU056828 | TCCTACGGGAGGCAGCAGT | GGACTACCAGGGTATCTAATCCTGTT |
Tricococcus sp. 16S rRNA | N/A | EU418263 | TCCTACGGGAGGCAGCAGT | GGACTACCAGGGTATCTAATCCTGTT |
Gene | MLST Allele | Genbank | Sequencing Forward 5′-3′ | Sequencing Reverse 5′-3′ |
aspA | 24 | EU056829 | CCAACTGCAAGATGCTGTACC | TTCATTTGCGGTAATACCATC. |
glnA | 2 | EU056830 | CATGCAATCAATGAAGAAAC | TTCCATAAGCTCATATGAAC |
gltA | 2 | EU056831 | CTTATATTGATGGAGAAAATGG | CCAAAGCGCACCAATACCTG |
glyA | 15 | EU056832 | AGCCTAATTCAGGTTCTCAA | AGGTGATTATCCGTTCCATCGC |
pgm | 294 | EU056833 | GGTTTTAGATGTGGCTCATG | TCCAGAATAGCGAAATAAGG |
tkt | 3 | EU056834 | GCTTAGCAGATATTTTAAGTG | AAGCCTGCTTGTTCTTTGGC |
uncA | 12 | EU056835 | AAAGTACAGTGGCACAAGTGG | TGCCTCATCTAAATCACTAGC |
C. jejuni 16S rRNA | N/A | EU056828 | TCCTACGGGAGGCAGCAGT | GGACTACCAGGGTATCTAATCCTGTT |
Tricococcus sp. 16S rRNA | N/A | EU418263 | TCCTACGGGAGGCAGCAGT | GGACTACCAGGGTATCTAATCCTGTT |
All amplicons from C.jejuni isolate unless stated otherwise.