Table 1. Conditions for RT-PCR quantification of specific mRNAs and 28S-rRNA.

RNA	Forward primer (5′→3′)	Reverse primer (5′→3′)	Tannealing (°C)	No. of cycles	MgCl2 (mM)
Murine
Adipoq mRNA	GGTGAGACAGGAGATGTTGG	CCTGATACTGGTCGTAGG TG	64	45	3
Lep mRNA	ACATTTCACACACGCAGTCG	AGC ATT CAG GGC TAA CAT CC	69	55	5
Il6 mRNA	GATGCTACCAAACTGGATATAATC	GGTCCTTAGCCACTCCTTCTGTG	65	45	6
Tnf mRNA	AAATGGCCTCCCTCTCATCA	AGATAGCAAATCGGCTGACG	63	45	3
Rbp4 mRNA	GTTTTCTCGTGACCCCAATG	GGAGGTGGGGGAAACTAAAC	68	50	3
28S-rRNA	CCAGTACTTCACTCCTGTCT	TCTAAGAGTGAGCAACGACG	61	45	3
Human
ADIPOQ mRNA	GTGATGGCAGAGATGGCAC	AGAGGCTGACCTTCACATCC	65	45	4
IL6 mRNA	CCAGCTATGAACTCCTTCTC	GCTTGTTCCTCACATCTCTC	63	45	3
TNF mRNA	GCGTGGAGCTGAGAGATAAC	GATGTTCGTCCTCCTCACAG	61	45	4
IL1B mRNA*	CATGGACAAGCTGAGGAAGA	TTCAACACGCAGGACAGGTA	61	45	4
PBEF1 mRNA	TCCAGGAAGCCAAAGATGTC	GGCCACTGTGATTGGATACC	61	40	3
RETN mRNA	GCCGGATTTGGTTAGCTGAG	CTCATTGATGGCTTCTTCCA	66	40	6
CCL2 mRNA	GCCTCCAGCATGAAAGTCTC	TGGAATCCTGAACCCACTTC	61	40	3
28S-rRNA	ACG GCG GGA GTA ACT ATG ACT	CTT GGC TGT GGT TTC GCT	63	50	4

^*For detection of IL1B mRNA, the fluorescent dye-linked probes 5′-AGGTGCTCAGGTCATTCTCCTGG-FL-3′ and 5′-Red640-AGGTCTGTGGGCAGGGAACC-PH-3′ were used instead of SYBR Green.