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(A) Schematic describing the process of preparation, processing, and quantification of the bronchiolar organ cultures. (B) The state of differentiation of airway epithelial cells was only minimally affected by maintaining bronchioles in cultures for 7 days, compared with bronchioles in in vivo lung. The numbers of ciliated and nonciliated cells were compared in bronchioles maintained in organ culture (OC) to the numbers in bronchioles of similar size in in vivo lungs (Lung).
