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. 2007 Sep 27;38(3):310–317. doi: 10.1165/rcmb.2007-0078OC

Figure 5.

Figure 5.

Resolution of IL-9/IL-13–induced MCM is dependent on Bax. (A) Representative figure showing a PCR analysis of genomic DNA from bax+/+, bax+/−, and bax−/− mice. (B) Bronchioles were microdissected from bax+/+ and bax−/− mice, placed in culture, and treated with IL-9 and IL-13 for 6 days, followed by 50 ng/ml IFN-γ treatment on Day 7. (C) Bronchioles were harvested and processed for quantification of MCM. Lanes 1 and 4 represent proteins from explants that were from bax+/+ or bax−/− mice, respectively. Bronchioles from bax−/− mice were infected with adenoviral expression system for Bax (lane 3) or green fluorescent protein (GFP) (lane 2) as control, harvested 2 days later, and analyzed by Western blotting. Hemagglutinin (HA)-tagged Bax was detected only in bronchioles infected with Ad-Bax. (D) Bronchioles from bax+/+ and bax−/− mice were placed in culture, treated with IL-9/IL-13 at 1 ng/ml each, and infected with Ad-Bax or Ad-GFP. Bronchioles were harvested, processed for AB/PAS staining, and analyzed for MCM. Error bars, group means ± SEM (n = 4 mice/group). Asterisk represents a significant difference (P < 0.05).