Figure 6.

Mice instilled with allergen for 5 days were infected with Ad-GFP or Ad-Bax. (A) Western blot analysis showing expression of HA-tagged Bax in 2 representative lungs of Ad-Bax- (lanes 1 and 2) but not in Ad-GFP–instilled (lanes 3 and 4) mice. A protein that cross-reacts with the HA-tag antibody was visible in all lanes. HA was detected by immunohistochemistry in epithelial cells of Ad-Bax– (B) but not Ad-GFP (C)–instilled mice. (D) MCM was significantly reduced in the airways of mice instilled with Ad-Bax compared with those instilled with Ad-GFP. Error bars, group means ± SEM (n = 4 mice/group). *Significantly different from Ad-Bax (P < 0.05).