Figure 4.

CINC-1 secretion by rat alveolar epithelial cells. Rat alveolar type I–like epithelial cells were cultured as described in Figure 2, and left untreated or pretreated with IL-1 antagonist (10 μg/ml) for 1 hour and then incubated with conditioned media. Media were collected 24 hours later and CINC-1 measured by enzyme-linked immunosorbent assay. A shows the results with NR8383-conditioned media and B shows the results with primary rat alveolar macrophage–conditioned media. The asterisk signifies inhibition compared with the respective control (P < 0.05).