Figure 5.

Macrophage IL-1α is primarily responsible for CINC-1 production from rat alveolar type I–like epithelial cells. Rat alveolar type I–like cells were cultured as described in Figure 2. Conditioned media generated from ozone-exposed macrophages were pretreated with IL-1α–selective neutralizing antibody (IL-1α NA), IL-1β NA, or control isotype match IgG at concentration of 3 μg/ml for 1 hour at room temperature before treatment of cultured cells. IL-1α NA alone as well as in combination with IL-1β NA significantly inhibited CINC-1 secretion compared with the IgG control condition (P < 0.05). In contrast, administration of IL-1β NA had no significant effect on CINC-1 secretion in these cultures.