FIG. 7.
Antiviral activity of nucleoside analogues against a high-fidelity PV variant (PV-G64S). Infections were performed at an MOI of 5 and treatment with 2 mM nucleoside analogue. Infection was allowed to proceed for 6 h at 37°C, and cell-associated virus was collected by freeze-thaw. (A) The titer of virus was determined on HeLa S3 cell monolayers. Data were normalized for each virus such that the virus titer resulting from untreated control infection was set equal to 100. Data are plotted as the means and standard deviations from three independent samples. (B) Virus was assayed for the Guar phenotype as described in the text. Results are reported as means and standard deviations from three independent samples. Due to the small amount of viable virus recovered from JA30-treated samples, n = 2 for wild-type (WT) PV and n = 1 for PV-G64S for this treatment only.