TABLE 3.
Nucleotide analogue incorporation into RNA by PV 3Dpol in vitro
| Nucleotide or analogue | Relative incorporation with templating basea:
|
|||
|---|---|---|---|---|
| Uridine
|
Cytidine
|
|||
| 60 s | 330 s | 60 s | 330 s | |
| JA23-TP | 0.54 | 0.96 | 0.1 | 0.15 |
| JA24-TP | 0.9 | 0.92 | 0.92 | 0.9 |
| JA26-TP | 0.79 | 0.79 | 0.18 | 0.25 |
| JA27-TP | 0.54 | 0.89 | 0.02 | 0.01 |
| JA28-TP | 0.92 | 0.82 | 0.94 | 0.9 |
| JA30-TP | 0.89 | 0.8 | 0.65 | 0.65 |
| JA31-TP | 0.92 | 0.79 | 0.31 | 0.65 |
| RTP | 0.13 | 0.57 | − | 0.6 |
| ATP | 1 | 1 | − | − |
| GTP | 0.13 | 0.61 | 1 | 1 |
a
Incorporation of analogues was measured by primer extension using PV 3Dpol. For each substrate, incorporation of the correct nucleotide was set to 1. −, no extension detected. Results for analogues capable of inducing transition mutations (Table 2) are shown in bold.