TABLE 2.
Primers and fluorescent probes used for real-time RT-PCR
| Gene (accession no.) | Primer or probe | Sequencea | Gene location (5′-3′) |
|---|---|---|---|
| CDR1 (X77589) | CDR1a | AACCGTTTACGTTGAACACGATAT | 2504-2527 |
| CDR1b | ACCAACTTCACCATCTTCAATGAC | 2565-2588 | |
| CDR1pr | 6FAM-ACTCACGCCGACACCACCGTTGTT-TAMRA | 2535-2558 | |
| CDR2 (U63812) | CDR2a | TGGCTAGTGTTTATATGGCAACCT | 1725-1748 |
| CDR2b | AAGCTTCAGCAATTGACACTCTTT | 1821-1844 | |
| CDR2pr | 6FAM-TCACCACCGGAAACACCACGCACA-TAMRA | 1792-1815 | |
| MDR1 (Y14703) | MDR1a | TCTCGGGTGGATTCTTTGCTAAT | 2659-2681 |
| MDR1b | AATGGACCAAAACTAGGACCACA | 2775-2797 | |
| MDR1pr | 6FAM-ACGGCACCCAAACTCCAAGCGGC-TAMRA | 2751-2773 | |
| FLU1 (AF188621) | FLU1a | TTTGTCGTTTCTTTGCTGGGTTT | 1153-1175 |
| FLU1b | ATTAAACATATCGGCCATAACGGC | 1206-1229 | |
| FLU1pr | 6FAM-AGCCACAACCAATGGAGCAGCACC-TAMRA | 1179-1202 | |
| ERG11 (AY856351) | ERG11a | TTATTAGGGGTTCCATTTGTTTACA | 76-100 |
| ERG11b | AAATTCATAAGGTTGTTGACCATATG | 188-213 | |
| ERG11pr | 6FAM-TGCAGAACCAAACCAAGGAATCCA-TAMRA | 160-183 | |
| TEF3 (Z12822) | TEF3a | AACCGTTTACGTTGAACACGATAT | 2480-2504 |
| TEF3b | ACCAACTTCACCATCTTCAATGAC | 2565-2588 | |
| TEF3pr | Texas Red-ACTCACGCCGACACCACCGTTGTT-BHQ2 | 2535-2558 |
a
Abbreviations: 6FAM, 6-carboxyfluorescein; TAMRA, 6-carboxy-N,N,N′,N′-tetramethylrhodamine; Texas Red, trademarked product from Molecular Probes; BHQ2, Black Hole Quencher 2.