Figure 1.
SDS/PAGE gels and autoradiograms of wild-type (WT) and its mutant HMMs. Samples of the purified HMMs were subjected to SDS/PAGE on 8 and 15% discontinuous acrylamide gels. Gels were stained with Coomassie brilliant blue (A). Light chain phosphorylation was performed in the presence (+) or absence (−) of myosin light chain kinase, calmodulin, and Ca2+ for 30 min. Samples were analyzed by SDS/PAGE on 15% gels and then subjected to autoradiography (B). HC, HMM heavy chain; LC20, regulatory light chain; and LC17, essential light chain.