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Initial phosphate bursts in the hydrolysis of ATP by wild-type and five mutant HMMs. Assay conditions were 0.20 mg/ml of each of wild-type (○), E470A (□), R247A (⋄), E470R (▵), R247E (▿), and E470R/R247E (•) HMMs, 0.45 M KCl, 2 mM MgCl2, 20 mM Tris⋅HCl (pH 7.5), 0.5 mM DTT, and 4 μM [γ-32P]ATP. Burst sizes for wild-type, E470A, R247, E470R, R247E, and E470R/R247E HMMs were 0.68, 0.00, 0.00, 0.00, 0.00, and 0.70 mol of Pi/mol of HMM head, respectively.
