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NMR diffusion and pH titration results. The chemical shifts of the Hɛ1 proton on His40 decrease distinctively at higher pH values (triangles). This matches the increase in the hydrodynamic radius (Å) obtained from the NMR diffusion experiment (diamonds), and the pH of the transition is in excellent agreement for both experiments. The dotted lines represent the theoretical hydrodynamic radii of completely monomeric (bottom) and completely dimeric (top) MIP-3α.
