FIG. 6.

EGCG damages HSV-1 virions. (a) Purified virus (strain F1) was incubated in tissue culture medium for 2 h at 37°C and then prepared for EM (see Materials and Methods). (b) Purified virus was incubated with EGCG (100 μM) for 2 h and then prepared for EM. (c) Purified virions were incubated with monoclonal antibody specific for the gD envelope glycoprotein (clone 1103, 67 μg/ml) and labeled with immunogold (see Materials and Methods). (d) Purified virions were incubated with EGCG (100 μM) for 2 h and treated as for panel C. Scale bars are 240 nm. The inserts in the upper right corners of panels a and b show representative virions magnified ×1.6.