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Effect of divalent cations on the killing of E. coli and C. albicans by the RTDs and PG-1. The killing of E. coli ML35 and C. albicans was measured by incubating increasing concentrations of RTD 1-3 with log-phase organisms in 10 mM PIPES, pH 7.4, with 1.2 mM CaCl2 and 0.8 mM MgCl2 or in 10 mM PIPES lacking added divalent salts.
