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Hemolytic activities of the RTDs and PG-1. Human red blood cells were incubated with 0 to 100 μg/ml of RTD 1, aRTD-1, and PG-1 in PBS for 1 h at 37°C. The absorbance of supernatants from the incubation mixtures was measured at 405 nm, and the percent hemolysis was calculated relative to the 100% hemolysis produced by 1% Nonidet P-40.
