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PLC-induced hydrolysis of NPPC by either 50 μl (⧫) or 100 μl (•) of an unconcentrated stationary-phase culture supernatant from the gatifloxacin-resistant mutant of C. perfringens (10G), 100 μl (▪) of the identically prepared culture supernatant from the WT C. perfringens strain, or 1 U (▴) of rPLC.
