TABLE 1.

Inhibition of C. parvum forms in cell culture by prophylactic and therapeutic treatments with Bobel-24

Treatment	Concn	Parasite counta	Growth inhibition
			%	Score
Bobel-24
Prophylactic treatment against sporozoitesb	90 μM	14.11 ± 2.28	27.8	0
Prophylactic treatment against cellsc	90 μM	12.50 ± 2.18	36	1
Therapeutic treatment against DEd	90 μM	0.43 ± 0.02	99.6	4
PRM	2 mg/ml	0.89 ± 0.02	95.4	4
Mucin	0.2 mg/ml	7.75 ± 0.39	60.4	2
Medium + DMSO	0.02%	15.31 ± 2.11	21.7	0
Medium		19.56 ± 1.78	0	0

^aShown is the mean number of DE per field ± the standard deviation. Values were determined by counting meronts and gamonts (<3 μm) to avoid counting nonviable but adherent sporozoites and merozoites.

^bSporozoite suspension incubated with Bobel-24 at 37°C for 30 min before use.

^cHCT-8 cells incubated with Bobel-24 at 37°C for 30 min before infection.

^dTreatment of infected HCT-8 cells with Bobel-24 at 37°C for 24 h in 5% CO₂.