TABLE 2.

Oligonucleotide primers used in this study to create NS3 protease mutant replicons by site-directed mutagenesis

Mutation	Original sequence (5′-3′)a	Primer (5′-3′)b	Strandc
V36M	GG GAG GTT CAA GTG GTC TCC ACC GC	GGGAGGTTCAAATGGTCTCCACCGC	S
		GCGGTGGAGACCATTTGAACCTCCC	AS
T54A	GC GTA TGT TGG ACT GTC TAC CAT GG	GCGTATGTTGGGCTGTCTACCATGG	S
		CCATGGTAGACAGCCCAACATACGC	AS
R155K	G GGC GTC TTC CGG GCC GCT GTA TGC	GGGCGTCTTCAAGGCCGCTGTATGC	S
		GCATACAGCGGCCTTGAAGACGCCC	AS
A156S	GC GTC TTC CGG GCC GCT GTA TGC AC	GCGTCTTCCGGTCCGCTGTATGCAC	S
		GTGCATACAGCGGACCGGAAGACGC	AS
A156V	GC GTC TTC CGG GCC GCT GTA TGC AC	GCGTCTTCCGGGTCGCTGTATGCAC	S
		GTGCATACAGCGACCCGGAAGACGC	AS
D168A	GCA AAG GCG GTG GAT TTT GTC CCC G	GCAAAGGCGGTGGCTTTTGTCCCCG	S
		CGGGGACAAAAGCCACCGCCTTTGC	AS
V170A	CG GTG GAT TTT GTC CCC GTT GAG TC	CGGTGGATTTTGCCCCCGTTGAGTC	S
		GACTCAACGGGGGCAAAATCCACCG	AS

^aBold indicates the codons to be mutated.

^bThe mutagenesis sites are underlined.

^cS, sense; AS, antisense.