TABLE 2.

List of extracellular proteins identified in filtered supernatants of MR-1 chemostat cultures grown with 0.1 mM P (PO₄)

Locus taga	Functional annotation	Predicted localization	Peptide countb
SO0189	Outer membrane fibronectin type III/cadherin domain protein	Outer membrane; β-barrel protein	3
SO0990	Periplasmic amidohydrolase 3 family protein	Periplasm	12
SO1221	Purine nucleoside phosphorylase (DeoD)	Cytoplasm	4
SO1557	Outer membrane porin	Outer membrane; β-barrel protein	20
SO1560	Phosphate-binding protein	Periplasm	23
SO1779	Outer membrane decaheme cytochrome c (OmcA)	Outer membrane; lipoprotein	5
SO1822	TonB-dependent receptor	Outer membrane; β-barrel protein	11
SO1844	Outer membrane endonuclease/exonuclease/phosphatase	Outer membrane; β-barrel protein	82
SO2001	Bifunctional UDP-sugar hydrolase/5′ nucleotidase (UshA)	Outer membrane; lipoprotein	30
SO2385	Monomeric alkaline phosphatase (PhoX)	Extracellular	35
SO2903	Cysteine synthase A (CysK)	Cytoplasm	8
SO2907	TonB-dependent receptor	Outer membrane; β-barrel protein	14
SO3237	Flagellin (FliC1)	Extracellular; flagella	22
SO3238	Flagellin (FliC2)	Extracellular; flagella	5
SO3247	Flagellar hook protein (FlgE)	Extracellular; flagella	11
SO3545	Outer membrane porin	Outer membrane; β-barrel protein	4
SO3565	Bifunctional 2′,3′ cyclic nucleotide 2′ phosphodiesterase/3′ nucleotidase (CpdB)	Outer membrane; lipoprotein	9
SO3896	Outer membrane porin (Omp35)	Outer membrane; β-barrel protein	8
SO4317	Cell surface calcium-binding protein with bacterial neuraminidase repeats	Extracellular	11
SO4652	ABC sulfate/thiosulfate transporter, periplasmic ligand-binding subunit (Sbp)	Periplasm	16
SO4719	ABC tungstate transporter, periplasmic ligand-binding subunit (TupA)	Periplasm	8
SOA0110	Expressed lipoprotein	Outer membrane; lipoprotein	5

^aLocus tag numbers correspond to the NCBI S. oneidensis MR-1 genome annotation (http://www.ncbi.nlm.nih.gov).

^bNumber of peptides detected for a particular protein. Only nonoverlapping, fully tryptic peptides are included.