FIG. 1.
Multiple alignment of protein sequences of thermostable pectate lyases and thermolabile PLXc in family PL1. The signal peptide sequences of the respective proteins are not shown. The numbering of each sequence starts from the initiation codon. Conserved catalytic sites (K199, R230, R235), conserved calcium binding sites (D137, D175, D179), the core structure of the parallel β-helix (vWiDH region), and sites conserved in all thermostable PL1 pectate lyases (CAD56882 from Bacillus licheniformis, BAA96478 from Bacillus sp. strain P-4-N, BAB40336 from Bacillus sp. strain TS-47, AAD35518 from Thermotoga maritima MSB8) but variant in PLXc (NP_638163) are shaded.