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Kinetic parameters of the parent PLXc and variants with PGA as the substrate
| Enzyme | Km (g·liter−1) | kcat (s−1) | kcat/Km (liter·g−1·s−1) | Sp act (U/mg) |
|---|---|---|---|---|
| Parent | 0.98 ± 0.20 | 114 ± 11 | 116 ± 17 | 196 ± 20 |
| A31G | 0.73 ± 0.11 | 194 ± 29 | 266 ± 40 | 333 ± 53 |
| R236F | 0.83 ± 0.15 | 121 ± 7 | 146 ± 16 | 208 ± 27 |
| A31G R236F | 0.93 ± 0.19 | 216 ± 12 | 232 ± 19 | 370 ± 22 |
