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Kinetic parameters of the variant pectate lyases with citrus pectin as the substrate
| Enzyme | Km (g·liter−1) | kcat (s−1) | kcat/Km (liter·g−1·s−1) | Sp act (IU/mg) |
|---|---|---|---|---|
| Parent | 0.80 ± 0.12 | 61 ± 7 | 76 ± 9 | 105 ± 12 |
| A31G | 0.68 ± 0.10 | 117 ± 10 | 172 ± 21 | 200 ± 25 |
| R236F | 0.59 ± 0.08 | 67 ± 7 | 114 ± 12 | 115 ± 15 |
| A31G R236F | 0.55 ± 0.07 | 139 ± 11 | 253 ± 30 | 238 ± 18 |
