TABLE 1.
E. coli strains used in this study
| Strain | Relevant genotype | Relevant phenotype | Reference |
|---|---|---|---|
| W3110 | Wild type (K-12 derivative) | Intermediate copper resistant | 1 |
| W3110(pPA173) | pcoABCDRSE from plasmid pRJ1004 | Copper resistant | 5 |
| GR5 | W3110 ΔcusCFBAa | Intermediate copper resistant | 12 |
| GR1 | W3110 ΔcueOa | Intermediate copper sensitive | 12 |
| EC949 | W3110 ΔcopA | Intermediate copper sensitive | This study |
| GR15 | W3110 ΔcusCFBA ΔcueOa | Intermediate copper sensitive | 12 |
| GR17 | W3110 ΔcusCFBA ΔcopA ΔcueOa | Copper sensitive | 12 |
a
Resistance cassette of the original strain was removed using plasmid pCP20 (7).