FIG. 1.

Rearrangement of the dsz operon by overlap PCR. Fragments of the 400-bp 5′ upstream segment (5′-U-S) and the 400-bp 3′ downstream segment (3′-D-S) of dszABC and the dszA and dszBC segments, including the overlap regions, were yielded by PCR, then the ligated 5′ upstream-dszBC segment (5′-U-S-dszBC) and the ligated dszA-3′ downstream segment (dszA-3′-D-S) were produced by overlap PCR via their overlap regions, and finally, the 5′ upstream-dszBC segment and the dszA-3′ downstream segment were linked together by overlap PCR via their overlap region to yield the reconstructed dsz operon. Black bars represent genes, and white bars represent overlap regions.