TABLE 1.
Culture growth conditions | Growth | Peak amt of DNA (ng) (SE) |
---|---|---|
Pyrite-enriched mine tailings | +++ | 3,610 (255) |
Fe(II) sorbed to ferrihydrite | +++ | 3,277 (53) |
Pyrite (FeS2)+ yeast extract (DSMZ 485 medium) | +++ | 3,244 (110) |
Pyrrhotite (FeS) | +++ | 2,779 (280) |
Pyrite (FeS2) + 20 mM Fe(II) | +++ | 2,592 (60) |
Elemental sulfur + 20 mM Fe(II) | +++ | 2,576 (313) |
Chalcopyrite (CuFeS2) | ++ | 2,140 (95) |
Pyrite (FeS2) + 20 mM Fe(II) | ++ | 1,807 (58) |
Elemental sulfur + 0.02% yeast extract | ++ | 1,609 (133) |
Covellite (CuS) | + | 1,000 (92) |
Sphalerite (ZnS) | + | 335 (54) |
Siderite (FeCO3) | − | NDb |
Magnetite (Fe3O4) | − | ND |
FeSO4 (20 mM) | − | NDc |
Tetrathionate (20 mM) | − | ND |
Na2S (5 μM) | − | ND |
Yeast extract (0.02%) in synthetic growth medium | − | ND |
Anaerobic (sulfur/ferrihydrite) | − | ND |
Anaerobic (pyrite/10 mM NO3−) | − | ND |
Anaerobic [Fe(II) ferrihydrite/10 mM NO3−) | − | ND |
Growth was assessed visually based on oxidation products (+++, substantial Fe(III) oxide production; −, no growth) and quantitatively after 11 days using total extractable DNA from 20-ml growth vessels (n = 3).
ND, not detected.
Abiotic oxidation of aqueous Fe(II) for >2 weeks resulted in the presence of Fe(III) oxide plus aqueous Fe(II), which could support growth of strain MK1.