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. 2007 Dec 14;74(4):942–949. doi: 10.1128/AEM.01200-07

TABLE 1.

Growth of Metallosphaera sp. strain MK1 in the presence of different minerals, substrates, or aqueous electron donors (pH 3, 65°C)a

Culture growth conditions Growth Peak amt of DNA (ng) (SE)
Pyrite-enriched mine tailings +++ 3,610 (255)
Fe(II) sorbed to ferrihydrite +++ 3,277 (53)
Pyrite (FeS2)+ yeast extract (DSMZ 485 medium) +++ 3,244 (110)
Pyrrhotite (FeS) +++ 2,779 (280)
Pyrite (FeS2) + 20 mM Fe(II) +++ 2,592 (60)
Elemental sulfur + 20 mM Fe(II) +++ 2,576 (313)
Chalcopyrite (CuFeS2) ++ 2,140 (95)
Pyrite (FeS2) + 20 mM Fe(II) ++ 1,807 (58)
Elemental sulfur + 0.02% yeast extract ++ 1,609 (133)
Covellite (CuS) + 1,000 (92)
Sphalerite (ZnS) + 335 (54)
Siderite (FeCO3) NDb
Magnetite (Fe3O4) ND
FeSO4 (20 mM) NDc
Tetrathionate (20 mM) ND
Na2S (5 μM) ND
Yeast extract (0.02%) in synthetic growth medium ND
Anaerobic (sulfur/ferrihydrite) ND
Anaerobic (pyrite/10 mM NO3) ND
Anaerobic [Fe(II) ferrihydrite/10 mM NO3) ND
a

Growth was assessed visually based on oxidation products (+++, substantial Fe(III) oxide production; −, no growth) and quantitatively after 11 days using total extractable DNA from 20-ml growth vessels (n = 3).

b

ND, not detected.

c

Abiotic oxidation of aqueous Fe(II) for >2 weeks resulted in the presence of Fe(III) oxide plus aqueous Fe(II), which could support growth of strain MK1.