FIG. 3.

Whole-cell N-glycan analysis of Aspergillus strains expressing engineered GNT I. (A, B, and C) The images on the left compare whole-cell N-glycans from A. nidulans strain YR23.5-BC10, which served as a recipient for the GNT I constructs (A) (shown in Fig. 1B, left, and repeated here for clarity), with strain YR23.5-BC10-coNA15 (B) and strain YR23.5-BC10-mnnJ-NA (C). The images on the right show whole-cell glycan spectra for A. niger strain T2-BC10, which served as a recipient strain for the GNT I constructs (A), compared to N-glycans obtained from strain T2-BC10-coNA15 (B) and strain T2-BC10-mnnJ-NA (C). (D) Schematic diagram representing the function of GNT I, which generates GlcNAcMan₅GlcNAc₂ structures by the addition of one GlcNAc moiety on Man₅GlcNAc₂. GlcNAc residues are represented by squares and mannose (hexose) residues by circles.