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Flow cytometric analysis of recombinant L. lactis IL 1403 cells. In all figures, gray histograms indicate results for control cells harboring pCUS, and white histograms indicate results for L. lactis IL 1403 cells harboring pMCS-AmyA (a), pCA-AmyA (b), pCA2-AmyA (c), pCA3-AmyA (d), and pAmyA-CA3 (e). Cells were labeled with primary rabbit anti-AmyA serum, followed by secondary goat anti-rabbit IgG conjugated with Alexa Fluor 488. For each experiment, 10,000 cells were analyzed.
