FIG. 3.

RT-PCR transcriptional analysis of the expression of cirA, flnA1, and flnE in Sphingomonas sp. strain LB126 during growth on fluorene or glucose. RT-PCR products obtained from template RNA extracted from cells grown on glucose (lanes G) and fluorene (lanes F) were separated by gel electrophoresis. The positions of size markers (indicated in base pairs) (lane M) are shown on the left (1-kb Plus DNA ladder; Invitrogen, Carlsbad, CA). Differentially amplified RT-PCR fragments indicate differential gene expression in fluorene-grown Sphingomonas sp. strain LB126 cells. Lane −, negative control without RNA but with 16S rRNA gene primers. RNA quality was checked by amplifying 16S rRNA gene sequences with RT and Taq polymerase.