TABLE 3.

Comparison of the FlnA1-FlnA2 dioxygenase activities toward fluorene and other polycyclic substrates^a

Substrate	Product retention time (min)	Product m/z	Product concn (μM)b
Fluorene	15.5	280	98
Phenanthrene	15.9	278	96
Anthracene	14.6	278	9
	16.6	278	27
Naphthalene	12.1	228	2
Dibenzofuran	13.5	418	10
Dibenzo-p-dioxin	14.1	434	10

^aCharacteristics of the NBB derivatives, except for dibenzofuran and dibenzo-p-dioxin, which were analyzed as the trimethylsilyl derivatives, are shown. Anthracene yielded two isomers, one of which was identified as anthracene 1,2-dihydrodiol (retention time, 16.6 min).

^bConcentrations in the bacterial suspension were calculated after 23 h of incubation at 25°C. Values are means of results from duplicate experiments. The standard error was less than 10%.