FIG. 3.

Evidence of adduct removal during production of LXR/RXR, elongin C, and the 18-kDa product. Cultures of the recombinant E. coli BL21(DE3)+PGL strain for production of LXR/RXR, elongin C, and 18-kDa protein were grown in parallel with corresponding “control” fermentations of the BL21(DE3) strain without PGL overexpression. The only difference between runs for product synthesis was the presence of the low-copy-number pECO-1pgl plasmid. As noted above, each comparative study included an assessment of the adduct levels by either LC/MS or high-resolution RP-HPLC analyses.