TABLE 2.
Effect of PGL coexpression on BL21(DE3) growth and the heterologously expressed 18-kDa protein
| Strain | Production 0 to 12 after induction
|
Production 12 to 24 h after induction
|
Final titers 24 h after induction
|
|||||
|---|---|---|---|---|---|---|---|---|
| Growth rate (1/h) | qGlc (g/h/g [dry wt]) | Yx/s (g/mol Glc) | Growth rate (1/h) | qGlc (g/h/g [dry wt]) | Yx/s (g/mol Glc) | Volumetric productivity (g/liter) | Specific productivity (mg/h/g [dry wt]) | |
| BL21 (PGL−) | 0.030 ± 0.002 | 1.41 ± 0.02 | 21.4 ± 0.1 | 0.011 ± 0.001 | 0.52 ± 0.02 | 19.2 ± 0.1 | 3.9 ± 0.1 | 1.82 |
| BL21 (PGL+) | 0.038 ± 0.001 | 1.47 ± 0.07 | 29.8 ± 0.4 | 0.016 ± 0.001 | 0.42 ± 0.09 | 30.7 ± 0.1 | 6.7 ± 0.6 | 2.88 |