TABLE 3.
Metabolic fluxes to the PP pathway exhibited by E. coli BL21(DE3) with and without PGL coexpression
| Strain | Period of induction (h) | Growth rate (1/h) | NADPH flux for anabolism (mmol/h/g [dry wt]) | Anabolic flux to PP pathway (mmol/h/g [dry wt]) | Catabolic flux to PP pathway (mmol/h/g [dry wt]) |
|---|---|---|---|---|---|
| BL21 (PGL+) | 0-12 | 0.038 | 0.618 (16)a | 0.044 (33) | 0.031 (41) |
| 12-24 | 0.016 | 0.301 (69) | 0.018 (63) | 0.001 | |
| BL21 (PGL−) | 0-12 | 0.030 | 0.534 | 0.033 | 0.022 |
| 12-24 | 0.011 | 0.178 | 0.011 | <0.001 |
a
The numbers in parentheses are percentages.