TABLE 1.
Physiological parameters of strains CRL264, CRL30, CRL30(pCIT), and CHCC2112 grown at a controlled pHa
| Strain | μmax ± SD (h−1) | OD600 ± SDb | GCR* (mmol Glc g biomass−1 h−1) | GCR (mmol Glc g biomass−1 h−1) | CCR (mmol cit g biomass−1 h−1) | YX/Glc (g biomass mol Glc−1) | YX/subst (g biomass mol C−1) | YX/ATP (g biomass mol ATP−1) | V (nmol citrate mg protein−1 min−1) |
|---|---|---|---|---|---|---|---|---|---|
| CRL264 | 0.59 ± 0.02 | 1.62 ± 0.01 | 12.0 | 7.7 | 33.8 | 30.5 | 17.8 | 16.8 | 4.8 |
| CRL30 | 0.49 ± 0.01 | 1.86 ± 0.15 | 11.1 | 0.0 | 23.6 | 23.5 | 12.2 | 0.3 | |
| CRL30(pCIT) | 0.49 ± 0.03 | 1.48 ± 0.05 | 9.4 | 7.1 | 14.9 | 31.6 | 17.2 | 15.4 | 1.9 |
| CHCC2112 | 0.44 ± 0.02 | 1.32 ± 0.10 | 8.9 | 5.5 | 27.8 | 35.1 | 18.0 | 22.5 | 1.3 |
Physiological parameters are shown for strains CRL264, CRL30, CRL30(pCIT), and CHCC2112 grown at a controlled pH of 4.5 in rich medium (M17′) supplemented with 55 mM glucose and 13.2 mM citrate. Mean values correspond to at least two independent determinations. Standard deviations (SD) for the μmax and OD are shown. GCR* is the average specific glucose consumption rate for the period of growth before citrate depletion, and GCR is for the entire growth phase; CCR, average specific citrate consumption rate; YX/Glc, biomass yield relative to glucose consumption; YX/subst, biomass yield relative to glucose plus citrate consumption; YX/ATP, biomass yield relative to ATP production; V, citrate uptake rate of resting cells (at 50 μM citrate).
OD600 values were calculated at the beginning of the stationary phase.