TABLE 2.

Growth parameters of strain CRL264^a

Growth conditions				μmax ± SD (h−1)	OD600 ± SD at stationary phase	CCR (mmol cit g biomass−1 h−1)	GCR* (mmol Glc g biomass−1 h−1)	GCR (mmol Glc g biomass−1 h−1)	YX/Glc* (g biomass mol Glc−1)	YX/Glc** (g biomass mol Glc−1)	YX/Glc (g biomass mol Glc−1)	YX/ATP (g biomass mol ATP−1)
Citrate content	pH controlled	pHin	pHfin
+	No	4.5	4.4	0.57 ± 0.02	1.33 ± 0.01	32.9	12.1	8.2	54.1	22.6	32.1	18.9
−	No	4.5	3.9	0.42 ± 0.002	0.81 ± 0.07		9.7b	6.8	38.8b	28.7b	31.1	15.6
+	Yes	4.5	4.5	0.59 ± 0.02	1.62 ± 0.01	33.8	12.0	7.7	64.8	19.9	30.5	16.8
−	Yes	4.5	4.5	0.45 ± 0.01	1.67 ± 0.01		10.5b	8.2	32.2b	23.2b	25.5	13.2
+	Yes	6.5	6.5	1.27 ± 0.02	5.75 ± 0.27	7.8	12.7	11.2	40.5	23.9	37.7	18.2
−	Yes	6.5	6.5	0.95 ± 0.01	6.90 ± 0.14		14.2b	12.6	54.0b	39.4b	45.7	20.3

^aGrowth parameters are shown for strain CRL264 grown in M17′ medium containing 55 mM glucose with (+) or without (−) 13.2 mM citrate under different pH conditions. pH_in, initial pH; pH_fin, pH value at the end of growth. The symbols * and ** indicate that the values were calculated during the time of growth up to citrate depletion or after citrate depletion, respectively. CCR, average specific citrate consumption rate; GCR, (without an asterisk) average specific glucose consumption rate for the entire growth phase. Y_X/Glc*, Y_X/Glc**, and Y_X/Glc indicate biomass yields relative to glucose consumption for the periods of growth before and after citrate depletion and for the entire growth phase, respectively. Y_X/ATP, biomass yield relative to ATP production. The data are mean values of at least two independent determinations. Standard deviations (SD) for μ_max and OD are presented.

^bValue calculated for the time period identical to that used for the corresponding cultivations with citrate.