TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s)a | Source or reference |
|---|---|---|
| Bacterial strains | ||
| E. coli XL1-Blue | recA1 endA1 hsdR Tcr | Stratagene |
| A. baylyi BD413 (ADP1) | Rifr mutant of strain BD4 (ATCC 33305) | 11 |
| A. baylyi BD413 aadA::gfp | A BD413 derivative containing, in the lipBA operon in the chromosome, an aadA::gfp fusion gene cassette; Rifr Kmr Spcr; GFP positive | This study |
| A. baylyi BD413(rbcL-ΔPaadA::gfp) | A BD413 derivative containing, in the lipBA operon in the chromosome, the cassette rbcL-ΔP-ΦaadA::gfp, with a transcriptional aadA::gfp fusion inactivation by a Prrn deletion; Rifr Kmr Spcs; GFP negative | This study |
| Bacillus subtilis TS116 | A PB168 derivative containing, in the chromosome, an aadA gene cassette between the tobacco genes rbcL and accD; Cmr Spcr | 2 |
| Azospirillum brasilense SPF94 (pRK290TS) | A derivative of strain SPF94 containing, on plasmid pRK290TS, an aadA gene cassette between the tobacco genes rbcL and accD; Rifr Spcr | 2 |
| Agrobacterium tumefaciens LBA 4404 (pRK290TS) | A derivative of strain LBA4404 containing, on plasmid pRK290TS, an aadA gene cassette between the tobacco genes rbcL and accD; Rifr Spcr | 2 |
| Sinorhizobium meliloti SPF94 (pRK290TS) | A derivative of the USDA1002 mutant strain containing, on plasmid pRK290TS, an aadA gene cassette between the tobacco genes rbcL and accD; Rifr Spcr | 2 |
| Plasmids | ||
| pCLT (also named pLEP01) | Cloning vector, containing the aadA gene cassette between rbcL and accD; Ampr; 7.0 kb | 12 |
| pZR80 | Integrative vector in the chromosomal lipBA operon of strain BD413 (ADP1); Ampr Kmr; 6.4 kb | 13 |
| pZR80-2 | pZR80 derivative, integrative vector at lipBA operon site; Kmr; 4.9 kb | This study |
| pZR80-2 aadA::gfp | pZR80-2 containing the fusion aadA::gfp; Kmr Spcr; 7.2 kb | This study |
| pZR80-2 (rbcL-ΔPaadA::gfp) | pZR80-2 with Prrn upstream of aadA::gfp deleted and with a 1.1-kb fragment containing rbcL; Kmr Spcs; GFP negative; 7.8 kb | This study |
| pRK290TS | A derivative of the broad-host-range vector pRK290; contains an aadA gene cassette between rbcL and accD; Tcr Spcr | 2 |
a
Amp, ampicillin; Cm, chloramphenicol; Rif, rifampin; Spc, spectinomycin; Tc, tetracycline.