TABLE 3.
Primers for PCR amplification of selected VNTR used in this study
| Locus | Primer | Primer sequence (5′-3′) | Product size (bp)a | Annealing temp (oC) |
|---|---|---|---|---|
| ESTR-1 | ESTR1-F | 5′ TET-TCCGGCCATTTCGCTCTG | 183 | 52 |
| ESTR1-R | 5′ TTACAGCGGCCCGAATACTC | |||
| ESTR-2 | ESTR2-F | 5′ FAM-AGGATAAAAATGCCGAGGGAATGT | 309 | 52 |
| ESTR2-R | 5′ TGGGCAACGATAAATCAAACACT | |||
| ESTR-3 | ESTR3-F | 5′ HEX-CGAAGACGGGTGATGAAAAA | 155 | 52 |
| ESTR3-R | 5′ CAGGCGAATTCCTCTCTGTT | |||
| ESTR-4 | ESTR4-F | 5′ HEX-GTGATACCGGCGTAATCCTG | 384 | 52 |
| ESTR4-R | 5′ GGCACCGCATAAAGAGAATG |
a
Size of product in absence of TR.