TABLE 1.

Primers used for saturation mutagenesis and sequencing of the V106 region in the tomA3 gene in TG1/pBS(Kan)TOM and the I100 region in the tmoA gene in TG1/pBS(Kan)T4MO

Primer	Nucleotide sequencea
Mutagenesis
TOM1	5′-CCGATGGAGAAAGTGTTTCCGTACGAC-3′
TOM2	5′-GTTGTAGTGCGAGAGAGCATGCATTTC-3′
TOM3	5′-CCTCGGTGTGCCATATACTCCAACGGTGTNNNACCCTGG-3′
TOM4	5′-GTCAACGCACTCAAGGTGTTCATCCAGGGTNNNACACCG-3′
T4MO_EcoRI_Front	5′-GGGAACAAAAGCTGGGTACCG-3′
T4MO_BglII_Rear_2	5′-TCCAAGCCCAGATCTATCAACGAGCGTTCG-3′
T4MO_100_Front	5′-ACTTTGAAATCCCATTACGGCGCCNNNGCAGTTGG-3′
T4MO_100_Rear	5′-GCTGCATATTCACCAACTGCNNNGGCGCCGTAATGG-3′
Sequencing
T4_100_Check	5′-GGATGCAGGTGCTTATTCGG-3′
TOM1	5′-CCGATGGAGAAAGTGTTTCCGTACGAC-3′

^aRestriction enzyme sites are underlined.