TABLE 2.
Apparent Vmax and Km values for E. coli TG1 expressing TOM and T4MO for oxidation of aryl alkyl sulfidesa
| Enzyme | Value for reaction:
|
|||||
|---|---|---|---|---|---|---|
| Thioanisole → methyl phenyl sulfoxide
|
Methyl-p-tolyl sulfide → methyl p-tolyl sulfoxide
|
|||||
| Vmax (nmol/min/mg protein) | Km (μM) | Vmax/Km | Vmax (nmol/min/mg protein) | Km (μM) | Vmax/Km | |
| TOM | 1.66 ± 0.49 | 129 ± 21 | 0.013 | 0.67 ± 019 | 68 ± 20 | 0.010 |
| T4MO | 0.85 ± 0.21 | 115 ± 28 | 0.007 | 0.070 ± 0.006 | 11 ± 4 | 0.006 |
a
Initial specific rates were determined for each reaction by monitoring the formation of the product using GC. Substrate concentrations were 0.1, 0.25, 0.50, 0.75, 1.0, 1.5, and 2.0 mM, and the cell OD was 11. Standard deviations are shown for two to three independent experiments.