TABLE 6.
Effects of MMM media compositions on hydrogen sulfide formationa
| Strain genotype | Effect of:
|
||||
|---|---|---|---|---|---|
| N at 433 mg/liter | N at 123 mg/liter | N at 65 mg/liter | N at 65 mg/liter plus 1/2 YNB | N at 123 mg/liter plus 1/5 YNB | |
| met17Δ | + | + | + | + | + |
| cys4Δ | + | + | + | + | + |
| gos1Δ | + | + | + | + | + |
| fcy22Δ | + | + | + | + | + |
| tpo2Δ | + | + | + | + | + |
| hom2Δ | + | + | + | + | + |
| hom6Δ | + | + | + | + | + |
| atp11Δ | + | + | 0 | 0 | + |
| ser33Δ | + | + | 0 | 0 | + |
| hht2Δ | + | 0 | 0 | + | tr |
| rxt2Δ | + | 0 | 0 | + | tr |
| iki3Δ | 0 | 0 | 0 | + | 0 |
| ypl035CΔ | 0 | 0 | 0 | tr | 0 |
| sit4Δ | tr | 0 | 0 | + | 0 |
| psy4Δ | 0 | tr | 0 | + | + |
| cgr1Δ | + | 0 | 0 | + | + |
H2S production was evaluated with lead acetate strips: +, a detectable amount of H2S was produced; 0, H2S was not detected; tr, very light color change indicating trace amounts of sulfide formation. The data represent the findings of at least two independent replicates.