FIG. 2.

Genes required for synthesis of TDA in TM1040. The black boxes indicate the ORF interrupted by the transposon. Arrows indicate ORFs transcriptional orientations, hatch marks indicate a break in the region, and the relative distance is indicated by the 1-kb marker. (A) tdaA∼tdaF genes reside on a plasmid, with their closest homologs found on the chromosome of P. denitrificans PD1222. An intergenic space of 54 bp separates TM1040 tdaA and tdaB, and 345 bp separate tdaB from tdaC-E, which overlap each other by one bp, and >10 kb separate tdaF from tdaE. (B) The remainder of the genes involved in TDA biosynthesis are located either on the chromosome (tdaH, malY, and cysI) or, in the case of the genes involved in phenylacetate catabolism (paaIJK), on plasmid pSTM1.