TABLE 2.
Microtiter test results
| Cosmid | Lipase productivity of strain with indicated cosmid ina:
|
||
|---|---|---|---|
| Medium 380 + soybean oil | Medium 380 + lactate | BHI + hexadecane | |
| 505 | 63.5 ± 10.4 | 28.8 ± 3.1 | 15.0 ± 3.6 |
| 71 | 40.3 ± 11.4 | 27.3 ± 5.3 | 16.7 ± 2.5 |
| 201 | 39.0 ± 5.6 | 18.0 ± 4.2 | 10.0 ± 1.4 |
| 726 | 36.8 ± 4.5 | 25.3 ± 4.9 | 21.0 ± 8.7 |
| pLAFR3 | 20.8 ± 2.1 | 11.5 ± 1.8 | 11.5 ± 2.9 |
a
Lipase productivity is the size of the clarification zone (mm2) on tributyrin oil agar plate/optical density at 600 nm for the 4 selected cosmid strains from the 20 lipase-stimulating strains chosen after microtiter assay done in quadruplicate.