TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Characteristics or synonym | Source or reference |
|---|---|---|
| Strains tested for N-AHSL degradation and presence of the qsdA gene | ||
| Actinoplanes utahensis NRRL 12052 | Source of aculeacin A deacylase | 39 |
| Corynebacterium glutamicum ATCC 14752 | Glutamine-producing strain | 38 |
| Escherichia coli Κ-12 | Lab collection | |
| Flavobacterium sp. strain ATCC 27551 | Parathion-degrading strain | 24 |
| Gordonia alkalivorans strain 98 | Petroleum-degrading strain | Lab collection |
| Microbacterium sp. strain POLB142 | Mannopine-degrading rhizospheric strain; soil isolate | 27 |
| Mycobacterium sp. strain Tn20 | Drought-resistant Sahara desert isolate | This study |
| Nocardia asteroides CECT 3051 | ATCC 19247; type strain | CECTa |
| Pseudonocardia autotrophica CECT 3044 | ATCC 13181; type strain | 47 |
| Ralstonia solanacearum GMI 1000 | Type strain | Lab collection |
| Rhodococcus erythropolis | ||
| W2 | N-AHSL-degrading isolate from soil | 44 |
| DCL14 | Limonene-degrading strain | 46 |
| SQ1 | ATCC 4277-1; R. erythropolis type strain | 33 |
| Mic1 | Mycorhizosphere isolate | Lab collection |
| MP50 | Aromatic-nitrile-degrading strain | 41 |
| CECT 3008 | ATCC 11048 | CECT |
| Rhodococcus fascians D188 | Fasciation-causing agent on dicotyledons | 3 |
| Rhodococcus opacus HL PM-1 | 2,4,6-Trinitrophenol-degrading strain | 25 |
| Rhodococcus rhodochrous CECT 3042 | ATCC 6846 | CECT |
| Rhodococcus corynebacteroides CECT 420 | ATCC 14898; soil isolate | CECT |
| Rhodococcus sp. strain RHA1 | Polychlorinated biphenyl-degrading strain | Lab collection |
| N-AHSL biosensors and N-AHSL-producing strains used for QQ assays | ||
| Agrobacterium tumefaciens NTL4(pZLR4) | Hydroxy, oxo, and long-chain N-AHSL sensor strain | 21 |
| Chromobacterium violaceum CV026 | Reduced N-AHSL sensor strain | 22 |
| Pectobacterium carotovorum Pcc797 | Soft-rot-causing agent | Lab collection |
| Pseudomonas fluorescens 1855-344 | Rhizospheric strain | Lab collection |
| Agrobacterium tumefaciens | ||
| 15955trac | Octopine-type strain, constitutive for Ti plasmid conjugal transfer | Lab collection |
| C58C1RS | Ti plasmid-cured C58 derivative resistant to rifampin and streptomycin | Lab collection |
| Escherichia coli strains used for cloning | ||
| DH5α | Lab collection | |
| VCS257 | Stratagene | |
| Plasmids | ||
| pUC19 | Ampr | Lab collection |
| pGEM-T | Ampr | Promega |
| pUC1318::Gm | Ampr Gmr | 40 |
| pME6032 | Tcr | 12 |
| pCP13/B | Tcr; cosmid vector | Lab collection |
| pSU16 | Tcr; cosmid clone conferring N-AHSL degradation capability | This study |
| pSU16-11 | pUC19 with a 3.2-kb EcoRI fragment from pSU16 conferring N-AHSL degradation | This study |
| pSU16-12 | pUC19 with a 3.5-kb BamHI fragment from pSU16 conferring N-AHSL degradation | This study |
| pSU40 | pME6032 harboring the 3.2-kb EcoRI fragment from pSU16-11 | This study |
| pSU8-1 | pGEM-T with a 1.8-kb PCR fragment containing orf1 and qsdA | This study |
| pSU8-1 ΔSalI | pSU8-1 with a SalI deletion in orf1 | This study |
| pSU8-1::Gm | pSU8-1 with insertion of a Gmr cassette at the AgeI site of qsdA | This study |
| pSU8-1 ΔSalI::Gm | pSU8-1 ΔSalI with insertion of a Gmr cassette at the AgeI site of qsdA | This study |
| pSU-qsdAW2 | qsdA ORF amplified and cloned into pET16b | This study |
| pSUreg1 | Upstream regulatory region of qsdA amplified and cloned into pGEM-T | This study |
a
CECT, Coleccion Española de Cultivos Tipo.