TABLE 2.
Distribution of number and type of editing in cob and cox1 mRNAs in dinoflagellates
| Edit | No. (%) in mRNA from the indicated speciesa
|
||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
cob
|
cox1d
|
||||||||||||||||
| Dac | Kbr | Pca | Pna | Ppi-like | Pre | Dac | Kbr | Kve | Pmic | Pmin | Ppi-like | Pre | Psh | Scrsp | Smi | Sysp | |
| Totalb | 31 (3.35) | 40 (4.30) | 32 (3.45) | 33 (3.54) | 32 (3.52) | 30 (3.22) | 30 (2.27) | 30 (2.27) | 39 (2.95) | 32 (2.42) | 34 (2.58) | 27 (2.05) | 27 (2.05) | 25 (1.89) | 28 (2.12) | 26 (1.97) | 28 (2.12) |
| A→G | 7 (22.6) | 11 (27.5) | 12 (37.5) | 13 (39.4) | 11 (34.4) | 11 (36.7) | 15 (50.0) | 16 (53.3) | 20 (51.3) | 15 (46.9) | 18 (52.9) | 13 (48.1) | 12 (44.4) | 12 (48.0) | 14 (50.0) | 18 (69.2) | 15 (53.9) |
| G→A | 1 (3.2) | 1 (2.5) | 0 (0) | 2 (6.1) | 1 (3.2) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 1 (4.0) | 0 (0) | 0 (0) | 0 (0) |
| C→U | 11 (35.5) | 11 (27.5) | 10 (31.3) | 9 (27.3) | 8 (25.0) | 8 (26.7) | 5 (16.7) | 3 (10.0) | 5 (12.8) | 3 (9.4) | 2 (5.9) | 3 (11.1) | 1 (3.7) | 2 (8.0) | 4 (14.3) | 2 (7.7) | 3 (10.7) |
| U→C | 9 (29.0) | 14 (35.0) | 8 (25.0) | 7 (21.2) | 8 (25.0) | 8 (26.7) | 5 (16.7) | 6 (20.0) | 9 (23.1) | 8 (25.0) | 8 (23.5) | 6 (22.2) | 7 (25.9) | 6 (24.0) | 6 (21.4) | 3 (11.5) | 9 (28.6) |
| U→G | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — |
| G→U | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — |
| U→Ac | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — |
| A→C | — | — | — | — | — | — | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 1 (3.8) | 1 (3.6) |
| G→C | 3 (9.7) | 3 (7.5) | 2 (6.3) | 2 (6.1) | 4 (12.5) | 3 (10.0) | 5 (16.7) | 5 (16.7) | 5 (12.8) | 6 (18.8) | 6 (17.6) | 5 (18.5) | 7 (25.9) | 4 (16.0) | 4 (14.3) | 2 (7.7) | 1 (3.6) |
| A, U→ G, C | 16 (51.6) | 25 (62.5) | 20 (60.6) | 19 (59.4) | 19 (63.3) | 20 (66.7) | 22 (73.3) | 29 (74.4) | 23 (71.9) | 26 (76.5) | 19 (70.4) | 19 (70.4) | 18 (72.0) | 20 (71.4) | 21 (80.8) | 24 (82.8) | |
Types of editing that are not detected in any species for an mRNA are denoted by dashes. Abbreviations of species names are as listed in Table 1.
Editing density.
One edit of this type has been documented in Ppi (21), but it is outside the region of sequence considered in this study (i.e., nt 88 to 1012 of Pfiesteria piscicida cob mRNA, GenBank accession no. AF357518 [for Amphidinium operculatum, the corresponding region is nt 126 to 1012]).
The region of sequence considered in this study is equivalent to Crypthecodinium cohnii cox1 genomic clone coding region nt 85 to 1404, GenBank accession no. AF186994 (for Amphidinium carterae and Amphidinium operculatum, the corresponding regions are nt 215 to 1404 and nt 274 to 1404, respectively).