FIG. 2.

Continuous biotransformation with fully induced E. coli JM101(pSPZ10) in a two-liquid-phase system. BEHP was used as the organic carrier solvent at a phase ratio of 1:1 in a 1-liter working volume. The dilution rates for the organic and aqueous phases were set at 0.1 h⁻¹. Styrene epoxidation was initiated at t = 0 by adding 60 mM of the inducer octane into the reactor and the organic-phase feed tank and 80 mM of styrene into the organic-phase feed tank. See Materials and Methods for further details. (A) Courses of biomass, glucose, and acetate concentrations in the aqueous phase and of styrene and styrene oxide concentrations in the organic phase. (B) Solid lines, experimental data; dotted lines, calculated wash-out kinetics after t = 22 h. Results were confirmed by an independent experiment.