TABLE 1.

Growth and biotransformation parameters during continuous cultivation of E. coli JM101(pSPZ10)^a

Steady stateb	Styrene feed concn (mM)	Cell concn (g CDW literaq−1)	Styrene oxide concn (mM)c	Styrene concn (mM)c	Specific epoxidation rate [U (g CDW)−1]	Acetate concn (g literaq−1)	Carbon balance (%)
							Biomass	Acetate	CO2	Sum
I	0	6.4				0.78	38	4	54	97
IId	0	6.1				0.78	36	4	57	97
III	39	5.9	19	1.4	5	0.76	35	4	60	99
IV	79	5.5	49	3.9	15	0.76	33	4	65	102
V	105	4.9	61	11	21	1.72	29	9	65	103
VI	131	4.8	68	26	24	1.97	28	10	66	104

^aThe dilution rates for the organic and aqueous phases were set at 0.1 h⁻¹. See Materials and Methods for details. The standard deviations of duplicate measurements were below 5%. aq, aqueous phase.

^bThe steady states refer to the experiment shown in Fig. 4.

^cConcentrations in the organic phase are given.

^dSteady-state II was established by adding the inducer DCPK to the bioreactor and the feed tank to a concentration of 1.7 mM in the organic phase.