FIG. 2.

Absorption spectra of the mixture of P252 and Chlide incubated under aerobic-light conditions. P252 at various concentrations (line 1, 0; line 2, 0.4; line 3,0.8; and line 4,1 μM) was incubated with 50 μM Chlide for 60 min under aerobic-light conditions at 25°C. All the samples were suspended in a total volume of 1 ml of 50 mM Tris-HCl with 100 mM NaCl, pH 8.0. After the reaction the mixture was subjected to absorbance scanning from 500 nm to 700 nm with a spectrophotometer. The experiment under aerobic conditions was done in an open-air bench in the laboratory under light at about 520 lx, equivalent to about 6,000 μmol m² s⁻¹.