TABLE 1.
E. coli strains used in this study
| Strain | Relevant genotypea | Source or reference |
|---|---|---|
| Strains used for construction | ||
| CC102 | F′CC102 | 9 |
| CC104 | F′CC104 | 9 |
| CC105 | F′CC105 | 9 |
| CC106 | F′CC106 | 9 |
| HC101 | polBex1 | 18 |
| KA796 | ara thi Δ(prolac) | 71 |
| MFH210 | polA1 zig-261::Tn10 | 25 |
| NR9163 | mutL218::Tn10 | 69 |
| NR9559 | mutL211::Tn5 | 14 |
| NR9695 | dnaQ49 zae-502::Tn10 | 70 |
| NR10173 | dnaE915 zae::Tn10d-cam | 14 |
| NR10775 | dnaE941 zae::Tn10d-cam zae-502::Tn10 | 67 |
| NR11258 | dnaE925 zae::Tn10d-cam | 14 |
| NR11916 | dnaX36 purE::Tn10 | 61 |
| NR12159 | dnaX36 zba-2321::mini-Tn10cam | 61 |
| NR13138 | ara, thi, Δ(prolac), trpE9777 | This study |
| PR68 | mutY68::Tn10kan | 63 |
| RW82 | Δ(umuDC)595::cat | 91 |
| SH2101 | ΔpolB1 [Δ(araD-polB)::Ω] | 11 |
| TT101 | mutM103::mini-tet | 54 |
| UTH2 | recA56 srl-360::Tn10 | 89 |
| YG7207 | Δ(dinB-yafN)::kan | 35 |
| Strains used for mutagenesisb | ||
| NR10452 | dnaE915, F′CC102 | This study |
| NR10454 | dnaE915, F′CC104 | This study |
| NR10455 | dnaE915, F′CC105 | This study |
| NR10456 | dnaE915, F′CC106 | This study |
| NR11102 | F′CC102 | This study |
| NR11104 | F′CC104 | This study |
| NR11105 | F′CC105 | This study |
| NR11106 | F′CC106 | This study |
| NR11172 | dnaE941, F′CC102 | This study |
| NR11174 | dnaE941, F′CC104 | This study |
| NR11175 | dnaE941, F′CC105 | This study |
| NR11176 | dnaE941, F′CC106 | This study |
| NR11939 | F′CC104 | This study |
| NR11940 | F′CC105 | This study |
| NR11951 | dnaX36, F′CC104 | This study |
| NR11952 | dnaX36, F′CC105 | This study |
| NR12193 | dnaQ49, F′CC104 | This study |
| NR12194 | dnaQ49, F′CC105 | This study |
| NR12197 | dnaX36 dnaQ49, F′CC104 | This study |
| NR12198 | dnaX36 dnaQ49, F′CC105 | This study |
| NR12526 | dnaX36 dnaE925, F′CC104 | This study |
| NR12527 | dnaX36 dnaE941, F′CC104 | This study |
| NR12528 | dnaX36 dnaE915, F′CC104 | This study |
| NR12529 | dnaX36 dnaE925, F′CC105 | This study |
| NR12530 | dnaX36 dnaE941, F′CC105 | This study |
| NR12531 | dnaX36 dnaE915, F′CC105 | This study |
| NR12532 | mutY, F′CC104 | This study |
| NR12533 | mutM, F′CC104 | This study |
| NR12534 | dnaX36 mutY, F′CC104 | This study |
| NR12535 | dnaX36 mutM, F′CC104 | This study |
| NR12548 | recA56, F′CC104 | This study |
| NR12549 | recA56, F′CC105 | This study |
| NR12550 | dnaX36 recA56, F′CC104 | This study |
| NR12551 | dnaX36 recA56, F′CC105 | This study |
| NR13153 | trpE9777, F′CC104 | This study |
| NR13155 | trpE9777, F′CC104/dinB | This study |
| NR13157 | trpE9777 dinB, F′CC104 | This study |
| NR13159 | trpE9777 dinB, F′CC104/dinB | This study |
| NR13256 | trpE9777 dnaX36, F′CC104 | This study |
| NR13258 | trpE9777 dnaX36, F′CC104/dinB | This study |
| NR13272 | trpE9777 dnaX36 dinB, F′CC104 | This study |
| NR13274 | trpE9777 dnaX36 dinB, F′CC104/dinB | This study |
| NR13276 | trpE9777 ΔumuDC, F′CC104 | This study |
| NR13278 | trpE9777 dnaX36 ΔumuDC, F′CC104/dinB | This study |
| NR13292 | trpE9777 dnaX36 ΔumuDC dinB, F′CC104 | This study |
| NR13296 | trpE9777 dnaX36 ΔumuDC dinB, F′CC104/dinB | This study |
| NR13312 | trpE9777 ΔumuDC, F′CC104 | This study |
| NR13314 | trpE9777 ΔumuDC, F′CC104/dinB | This study |
| NR13316 | trpE9777 ΔumuDC dinB, F′CC104 | This study |
| NR13318 | trpE9777 ΔumuDC dinB, F′CC104/dinB | This study |
| NR16108 | trpE9777 dnaX36 polBex1, F′CC104 | This study |
| NR16116 | trpE9777 dnaX36 polBex1 dinB, F′CC104/dinB | This study |
| NR16159 | trpE9777 dnaX36 ΔpolB, F′CC104 | This study |
| NR16163 | trpE9777 dnaX36 ΔpolB dinB, F′CC104/dinB | This study |
| NR16169 | trpE9777 dnaX36 polBex1, F′CC105 | This study |
| NR16173 | trpE9777 dnaX36 polBex1 dinB, F′CC105/dinB | This study |
| NR16176 | trpE9777 dnaX36 ΔpolB, F′CC105 | This study |
| NR16183 | trpE9777 dnaX36 ΔpolB dinB, F′CC105/dinB | This study |
| NR16226 | trpE9777, F′CC105 | This study |
| NR16228 | trpE9777 dinB, F′CC105/dinB | This study |
| NR16246 | trpE9777 dnaX36, F′CC105 | This study |
| NR16248 | trpE9777 dnaX36 dinB, F′CC105/dinB | This study |
| NR16878 | trpE9777 polBex1, F′CC104 | This study |
| NR16879 | trpE9777 polBex1, F′CC105 | This study |
| NR16889 | trpE9777 ΔpolB, F′CC104 | This study |
| NR16890 | trpE9777 ΔpolB, F′CC105 | This study |
| NR17223 | trpE9777 polBex1 dinB, F′CC104/dinB | This study |
| NR17224 | trpE9777 polBex1 dinB, F′CC105/dinB | This study |
| NR17225 | trpE9777 ΔpolB dinB, F′CC104/dinB | This study |
| NR17226 | trpE9777 ΔpolB dinB, F′CC105/dinB | This study |
| NR17502 | dnaE925, F′CC102 | This study |
| NR17504 | dnaE925, F′CC104 | This study |
| NR17505 | dnaE925, F′CC105 | This study |
| NR17506 | dnaE925, F′CC106 | This study |
| NR17507 | dnaQ930, F′CC104 | This study |
| NR17508 | dnaX36 dnaQ930, F′CC104 | This study |
| NR17509 | dnaQ930, F′CC105 | This study |
| NR17510 | dnaX36 dnaQ930, F′CC105 | This study |
a
F′CC102, F′CC104, F′CC105, and F′CC106 refer to the F′prolac originally present in strains CC102, CC104, CC105, and CC106, which permit measurement of lac G·C→A·T, G·C→T·A, A·T→T·A, and A·T→G·C base substitution mutations, respectively (9). The designations F′CC104/dinB and F′CC105/dinB indicate deletion of the dinB gene on the F′ episome. See Materials and Methods for details on the various constructions.
b
All of these strains are also ara thi mutL Δprolac.