TABLE 3.
Suppression of Mre− and MrdB− lethality by multiple copies of sdiA
| P1 lysatea | No. of colonies for:
|
|
|---|---|---|
| PB103/pGB2 (wt/vector) | PB103/pCX16 (wt/sdiA) | |
| lacIZYA<>aph | 326 | 496 |
| mreBCD<>aph | 1 | 149 |
| mreB<>aph | 1b | 133 |
| mreC<>aph | 1 | 123 |
| mreD<>aph | 0 | 110 |
| mrdB<>aph | 0 | 77 |
a
Equal aliquots of saturated cultures were mixed with equal aliquots of each transducing lysate, the mixtures were plated on LB-Kan-spectinomycin, and the numbers of transductant colonies that appeared after incubation at 37°C were counted.
b
This transductant yielded strain FB2sup/pGB2.