TABLE 2.
Primer | Sequence (5′-3′)a | Restriction site added |
---|---|---|
ftnA1s | GCTGCGTATGGTTATTTTCTG | |
ftnA1r | GAAATAATCGGCGTGTATCC | |
ftnA2s | CCATCAGTCACCTGCCAGTAA | |
ftnA2r | GGATAATCAGCCGCCCAGCAA | |
bfr1s | GGTCGTGTAGAGCGCGGCA | |
bfr1r | CAGTACATAACCCCCATAT | |
bfr2s | AAATGAGTTATCCGCATATGT | |
bfr2r | GTGCTGACGAACGACCTGTCG | |
ryhB1s | GTGGACCGCCTATACGCT | |
ryhB1r | CGCGGATCCGATGAGTCACAAGGATGG | BamHI |
ryhB2s | AGCGGATCCTGGTTTCCTATTTGTTTG | BamHI |
ryhB2r | TCCAAAACCCTGTCCGCC | |
rpoS5 | CGGGATCCGGATGATCGAGAGTAAC | BamHI |
rpoS6 | CGGGATCCTTCAACCTGAATCTGGC | BamHI |
rpoS1s | ATCACGGGTAGGAGCCACTTA | |
rpoS1r | TTATTGTGCGAGTTATTCGCG |
Underlining indicates the restriction site.